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Briefly, GSTO1-1 pQE30 plasmid was transformed into M15 cells, which were grown in Luria-Bertani broth supplemented with 100 μg ml −1 ampicillin and 25 μg ml −1 kanamycin at 37 °C to an OD 600 of ∼0.8 and induced with 0.1 mM isopropyl thio- β -D-galactoside for 4 h