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For the superior sagittal sinus pressure recording, we made a single burr hole in the rostral part of the sagittal suture, above the superior sagittal sinus, and cannulated the anterior portion of the superior sagittal sinus by Braun intravenous cannulas

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In saline-treated rats, early myocardial damage appeared by day 24, marked by a significant increase in left ventricular systolic diameter