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The cytosolic iron sensors IRP1, which senses iron levels through an iron−sulfur cluster, and IRP2, which senses iron levels through iron-dependent degradation by the hemerythrin-like ubiquitin ligase, FBXL5 (99−101), are activated, and they repress synthesis of proteins such as ferritin and ferroportin, which are encoded by transcripts that contain an IRP binding site in the 5′ UTR and increase the rate of synthesis of iron uptake proteins such as TfR1

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Animals were picked into 20 μL of M9 on slides and gently immobilised with a coverslip