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First, a proof-of-concept study was performed using pooled serum to identify the IgG N-glycan signature of healthy control, acute LD, and patient-matched antibiotic-treated LD serum using high-pressure liquid chromatography separation (HPLC) and detection of fluorescently labeled glycans ( Acute LD IgG N-glycans gain terminal galactose and sialic acid HPLC analysis of the glycan signature revealed several statistically significant differences between control and acutely infected patients ( Figure 1 )